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KMID : 0606920120200030320
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Biomolecules & Therapeutics
2012 Volume.20 No. 3 p.320 ~ p.325
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Expression of Recombinant Rotavirus Proteins Harboring Antigenic Epitopes of the Hepatitis A Virus Polyprotein in Insect Cells
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Than Van Thai
Baek In-Hyuk
Lee Hee-Young
Kim Jong-Bum
Shon Dong-Hwa
Chung In-Sik
Kim Won-Yong
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Abstract
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Rotavirus and hepatitis A virus (HAV) spread by the fecal-oral route and infections are important in public health, especially in developing countries. Here, two antigenic epitopes of the HAV polyprotein, domain 2 (D2) and domain 3 (D3), were recombined with rotavirus VP7, generating D2/VP7 and D3/VP7, cloned in a baculovirus expression system, and expressed in Spodoptera frugiperda 9 (Sf9) insect cells. All were highly expressed, with peak expression 2 days post-infection. Western blotting and ELISA revealed that two chimeric proteins were antigenic, but only D2/VP7 was immunogenic and elicited neutralizing antibody responses against rotavirus and HAV by neutralization assay, implicating D2/VP7 as a multivalent subunit-vaccine Candidate for preventing both rotavirus and HAV infections.
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KEYWORD
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Rotavirus, Hepatitis A virus, Recombinant chimera protein
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